An eyespot (sometimes ocellus) is an eye-like marking. They are found in butterflies, reptiles, cats, birds and fish.

Eyespots could be explained in at least three different ways. They may be a form of mimicry in which a spot on the body of an animal resembles an eye of a different animal, to deceive potential predator or prey species. They may be a form of self-mimicry, to draw a predator's attention away from the prey's most vulnerable body parts. Or they may serve to make the prey appear inedible or dangerous. Eyespot markings may play a role in intraspecies communication or courtship; the best-known example is probably the eyespots on a peacock's display feathers.

The pattern-forming biological process (morphogenesis) of eyespots in a wide variety of animals is controlled by a small number of genes active in embryonic development, including the genes called Engrailed, Distal-less, Hedgehog, Antennapedia, and the Notch signaling pathway.

Artificial eyespots have been shown to reduce predation of cattle by lions.